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April 2007 Archives

April 1, 2007

1st world waste

my friend bern sent me this link... and i am totally guilty of buying into the consumer culture that has surrounded us in the developed world (pardon the pun). there are times i look back and wish i was less like the self-centered "me first" person that i am. it's good to be reminded that people can make a difference if they would just cut back a little bit of greed and think about others. i've always respected and liked sarah mclachlan... this video reminds me again why.

April 4, 2007

last month at ub

so it's april, time goes by so quickly. just one more month officially at the university at buffalo. i'll be continuing my research and writing my thesis and defending over summer but i won't have to register for any classes. it seems that i just recently started grad school here, and yet it feels so far away at the same time. i think i've changed a little from these 3 years i've been back home. i'm a little more confident in what i'm doing, and why i've done what i've done. was a bit of a gamble coming back to school, didn't know if i'd be able to achieve my goals, but i'm glad i did it. talked to my advisor for my public health masters program, said i should take a leave of absence from that program. i'm only 4 courses, a couple weeks of field training experience, and a masters project away from earning that degree. i've decided on a pharmacy school too (despite my piscean nature of being indecisive). i'll be heading to northeastern university in boston this fall for the next 4 years. the school actually started a new track for graduate entry, which means i get to take electives in the graduate school along with the pharmacy school. my advisor noted that i could transfer 3 courses (up to 10 credits) in the grad school at northeastern, or take classes at the other 3 public health programs in boston (tufts, boston university, harvard) towards my mph program. also, since northeastern has co-ops on top of their pharmacy experiential year i should be able to get my field training waived if i complete a co-op that has to do with public health or upon finishing my experiential year. if all plays out i would only have to take 1 course (at ub) and complete my project and i'd also have another masters after pharm school.

so... other good news. been using silver staining on acrylamide gels in lab vs. agarose with ethidium bromide due to the small fragment i'm trying to cut out from my vector. these tiny 56 base pair fragments weren't showing up on the agarose gel due to the rest of the gel being lit up by the large sized pieces. on a bunch of my samples i saw that something was being cut and released, but couldn't see the smaller fragment. my biotech advisor/pi suggested switching to the acrylamide. the first couple i ran, the resolution wasn't great (and i totally ripped the gel on a couple) but it looked like something was there. on one i ran today, i saw a clear and consistent difference with banding between my uncut plasmid and my restriction digested ones. i've got 4 samples of 2 other different strands i ligated and transformed into e. coli to run tomorrow. hopefully i'll get similar results, culture and freeze down some cells, and send off a batch to get dna sequencing done. if this all works out (big if), i can start transfecting some mel cells with my vector to see if i can get this whole sirna thing to work out. this is the best news in lab i've gotten in a really long time. plus, i'll actually have some nice pictures of gels (results, finally!) to show during my department seminar on monday.

on top of all this good news... the sabres won tonight! they captured the eastern conference for the regular season (and home-ice throughout the eastern conference playoffs), as well as the northeast division! yay... new banners @ hsbc!. we're 1 point behind detroit for the president's trophy with one game in hand. detroit's won so many frickin times... i hope the little guy gets it this year (sorry k), though even if we don't end up with the president's trophy, i'd give that up for the cup in a heartbeat. also... the leafs are 1 point out of 8th, and i'd rather play toronto than montreal (sorry k... again) if i had a choice... we've owned the leafs for a very long time. the team i'm most scared of in the playoffs is jersey... they've always been tough for us to beat no matter what time of the year it is. also, in case you haven't read this... the sabres are ranked number 1 in the ultimate standings across all 4 major sporting leagues. buffalo also placed first with another dubious honor... this was with the bills (those scrubs). ranked number 1 on the pain and suffering index by espn. our 2 pro sports teams on the opposite ends of the spectrum. whatever... hockey is a much better sport =].

i've been really liking the mix pandora has been putting out for me lately. found a lot of new stuff that i'm digging.

so... i've been pretty happy lately which is a weird feeling. next week brings my seminar, an exam, and a term paper... and after that... it looks like smooth sailing school wise until summer starts. i'm looking forward to that.

haven't written a post this long in awhile. thanks for reading. have good hump days!

April 5, 2007


been listening to moby's album hotel, and some remixes of some song lately. digging the sound.

ok... office time... and sabres game on. my gel pics were met with approval from my adisor =]. what a good night.

April 7, 2007

A Reason to Post

Free Ice-Cream! For those of you living in CT, MA, ME, NH, NY, or RI, print this coupon and take it to your local Baskin-Robbins dealer to get your fix: one free scoop.

I'm recovering from a really nasty flu. Two Sundays ago, I noticed something in my throat, and Monday morning my doctor prescribed azithromycin. After a week, it didn't really go away, but it didn't really get worse either. I was tired, but I was able to sleep and fight it. I worked all through that week.

Then last Sunday, I started to feel an itch in my lungs, and by Monday there was heaviness and coughing. Went to see my doctor, and he prescribed a second round of zithro, and some promethazine syrup for the cough. He told me to stay home for a couple of days, which turned out to be a huge understatement. I could barely do anything for five days. I finally regained my energy a few hours ago.

I still have that nasty cough, but it's subsiding, plus I'm starting to get clogged sinuses, which usually occurs when the bug is on its way out. That means I'll have to pick up some pseudoephedrine tomorrow - it'll actually be the first time I do that since all this business about registering to purchase it due to the crystal meth craze. Maybe I'll try phenylephrine this time, which pharmaceutical companies have released as an alternative.

Anyway, I'm all achy, partly from the flu, and partly from lying down most of the week. But now it's time for bed. Hope y'all don't get this bug -- though I seem to have caught it later than most people I know. I have been reading Stephen King's The Stand, which at a certain point I decided might not be the best thing to read when trying to overcome a flu that won't die.

April 9, 2007

ok... so...

i know sometimes i post about what i'm doing in my lab, and your eyes may sort of glaze over and not know exactly what i'm saying, but... if you're interested this is in essence what i'm trying to do. i found a good site/video which explains the basic technique (RNAi) i'm trying to use to figure out the function of this mRNA sequence produced from a gene that had been discovered by previous members of my lab. for you science-interested types... hope you enjoy.

April 12, 2007

sabres over isles

woo hoo... sabres played a nice game tonight. only 15 more to go and our stanley cup drought will end.

for you leafs fans... check this out.

here's some more funny

April 17, 2007

Save Pandora & Internet Radio

Pandora is a really cool internet radio site that grew out of the Music Genome Project, which painstakingly analyzes as much rock music as it can. So what Pandora is able to do is create a play list of music for you based on songs that you tell it that you like. They're now in trouble. I'll let Pandora's founder explain the details:

Hi, it's Tim from Pandora,

I'm writing today to ask for your help. The survival of Pandora and all of Internet radio is in jeopardy because of a recent decision by the Copyright Royalty Board in Washington, DC to almost triple the licensing fees for Internet radio sites like Pandora. The new royalty rates are irrationally high, more than four times what satellite radio pays and broadcast radio doesn't pay these at all. Left unchanged, these new royalties will kill every Internet radio site, including Pandora.

In response to these new and unfair fees, we have formed the SaveNetRadio Coalition, a group that includes listeners, artists, labels and webcasters. I hope that you will consider joining us.

Please sign our petition urging your Congressional representative to act to save Internet radio: http://capwiz.com/saveinternetradio/issues/alert/?alertid=9631541

Please feel free to forward this link/email to your friends - the more petitioners we can get, the better.

Understand that we are fully supportive of paying royalties to the artists whose music we play, and have done so since our inception. As a former touring musician myself, I'm no stranger to the challenges facing working musicians. The issue we have with the recent ruling is that it puts the cost of streaming far out of the range of ANY webcaster's business potential.

I hope you'll take just a few minutes to sign our petition - it WILL make a difference. As a young industry, we do not have the lobbying power of the RIAA. You, our listeners, are by far our biggest and most influential allies.

As always, and now more than ever, thank you for your support.

-Tim Westergren
(Pandora founder)

I signed it, and if you agree with the petition I urge you to join it as well. Cheers for now.

April 23, 2007


it's been hard getting through these last 2 weeks or so. there've been some positive things happening, like the sabres getting into the 2nd round, it's finally warmed up significantly, got ice cream, i'm done with the majority of difficult exams, papers, presentations for the semester. however... this has all been tempered by a sinking feeling that's kinda taken hold. there are times when i wish i could disconnect myself from the world.

April 24, 2007

God Bless Roger Ebert

I don't think there's any film critic alive who loves movies more than Roger Ebert. He's had some tough times lately, but he's still going to attend his ninth annual overlooked films festival in Chicago. He has lost a lot of weight, and he can't speak at the moment because he is missing part of his jaw and has an air hole in his throat, but his spirit and good humor seems undimmed. Here's hoping he gets better and gets to tell us personally about all the great movies out there that we ought to explore and watch.

About April 2007

This page contains all entries posted to .:Gotta Drop a Blog in The Corner:. in April 2007. They are listed from oldest to newest.

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